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Title:	HPV genotyping and p16 expression in Xingu Indigenous Park, Brazil
Authors:	Freitas, Valéria Grisolia Focchi, Gustavo R. Pereira, Érica Ribeiro Levi, J.E. Speck, Neila M. de Góis Ribalta, Julisa Chamorro
Affilliation:	Universidade Federal de São Paulo. Departamento de Ginecologia. Núcleo de Prevenção de Doenças Ginecológicas. São Paulo, SP, Brasil Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Patologia. São Paulo, SP, Brasil Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Medicina Preventiva. São Paulo, SP, Brasil Universidade de São Paulo. Instituto de Medicina Tropical. São Paulo, SP, Brasil Universidade Federal de São Paulo. Departamento de Ginecologia. Núcleo de Prevenção de Doenças Ginecológicas. São Paulo, SP, Brasil Universidade Federal de São Paulo. Departamento de Ginecologia. Núcleo de Prevenção de Doenças Ginecológicas. São Paulo, SP, Brasil
Abstract:	The association between high-risk human papillomavirus (HPV) genotypes and p16 expression in indigenous women from the Xingu Indigenous Park, Brazil, was unknown. This study evaluated p16 expression in women with a histological diagnosis of cervical intraepithelial neoplasia (CIN) 3 or higher and correlated this expression with HPV genotypes to determine possible discrepancies in the expression of this marker. We evaluated 37 previously collected samples with different HPV genotypes and high-grade lesions diagnosed based on cytology, histology, and colposcopy. Immunohistochemical analysis was performed using paraffin-embedded tissue sections and the CINtec® Histology Kit. p16 protein expression was investigated by immunostaining with an anti-p16 antibody. HPV genotyping was performed by reverse hybridization. The age of the study population ranged from 22-75 years (43.81 ± 15.89 years) and parity ranged from 1-11 (5.92 ± 2.58). Thirteen different HPV genotypes were found using the INNO-LiPA kit. Single and multiple infections by HPV were found with prevalence of single infections (P = 0.029). Comparison between HPV genotype and simple or multiple infections was highly significant; it was observed more HPV 52 followed by HPV 16 in single infections (P < 0.001). p16 expression was predominantly diffuse, which was observed in 91.7% of lesions, whereas 8.3% were focal (P < 0.001). HPV 52, HPV 16 and 31 were the most prevalent HPV types in high-grade CIN in these indigenous women. Diffuse p16 expression in high-grade CIN was not influenced by the viral genotype; however, more studies are necessary to further our understanding of this restricted group.
Keywords:	Brazil Health of Indigenous Peoples Indians, South American Genotype Genes, p16 Alphapapillomavirus Women's Health
Keywords:	Mato Grosso Região Amazônica Região Centro-Oeste Xingú
DeCS:	Brasil Saúde de Populações Indígenas Índios Sul-Americanos Saúde da Mulher Genótipo Alphapapillomavirus Genes p16
Issue Date:	2016
Publisher:	Fundação de Pesquisas Cientificas de Ribeirão Preto
Citation:	FREITAS, V.G.; FOCCHI, G.R.; PEREIRA, E.R.; LEVI, J.E.; SPECK, N.M.G.; RIBALTA, J.C. HPV genotyping and p16 expression in Xingu Indigenous Park, Brazil. Genetics and Molecular Research, Ribeirão Preto, v. 15, n. 3, p. gmr.15036840, 2016.
metadata.dc.identifier.doi:	10.4238/gmr.15036840
Copyright:	open access
Appears in Collections:	DIP - Artigos de Periódicos

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